rs9390123 and rs9399451 influence the DNA repair capacity of lung cancer by regulating PEX3 and PHACTR2‑AS1 expression instead of PHACTR2

  • Authors:
    • Qiang Shi
    • Qiao-Na Shi
    • Jiang-Wei Xu
    • Hong-Yan Wang
    • Ya-Jie Li
    • Xin-Xin Zhang
    • Yu-Hang Fu
    • Ru-Hui Tian
    • Ru Jiang
    • Chun-Chun Liu
    • Chang Sun
  • View Affiliations

  • Published online on: January 21, 2022     https://doi.org/10.3892/or.2022.8270
  • Article Number: 59
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Abstract

Lung cancer is a common cancer type, and has the highest mortality rate in the world. A genome‑wide association study suggests that the genetic marker rs9390123 is significantly associated with DNA repair capacity (DRC) in lung cancer. Analysis of the data derived from the 1000 Genomes Project indicated that there is another single nucleotide polymorphism (SNP), rs9399451, in strong linkage disequilibrium with rs9390123 in Caucasian individuals, thus suggesting that this SNP could be associated with DRC. However, the causal SNP and mechanism of DRC remain unclear. In the present study, dual luciferase assay results indicated that both SNPs are functional in lung cells. Through chromosome conformation capture, an enhancer containing the two functional SNPs was observed to bind the promoter of peroxisomal biogenesis factor 3 and phosphatase and actin regulator 2 antisense RNA 1 (PHACTR2‑AS1). Knockdown of PHACTR2‑AS1 could significantly influence lung cell proliferation, colony formation, migration and wound healing, which verified that PHACTR2‑AS1 is a novel oncogene for lung cancer. Through chromatin immunoprecipitation, the transcription factor POU class 2 homeobox 1 (POU2F1) was identified to bind to the surrounding segments of these two SNPs, and their interaction was investigated. The present study identified the mechanism via which rs9390123 and rs9399451 could influence DRC.
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March-2022
Volume 47 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shi Q, Shi Q, Xu J, Wang H, Li Y, Zhang X, Fu Y, Tian R, Jiang R, Liu C, Liu C, et al: rs9390123 and rs9399451 influence the DNA repair capacity of lung cancer by regulating <em>PEX3</em> and <em>PHACTR2‑AS1</em> expression instead of <em>PHACTR2</em>. Oncol Rep 47: 59, 2022
APA
Shi, Q., Shi, Q., Xu, J., Wang, H., Li, Y., Zhang, X. ... Sun, C. (2022). rs9390123 and rs9399451 influence the DNA repair capacity of lung cancer by regulating <em>PEX3</em> and <em>PHACTR2‑AS1</em> expression instead of <em>PHACTR2</em>. Oncology Reports, 47, 59. https://doi.org/10.3892/or.2022.8270
MLA
Shi, Q., Shi, Q., Xu, J., Wang, H., Li, Y., Zhang, X., Fu, Y., Tian, R., Jiang, R., Liu, C., Sun, C."rs9390123 and rs9399451 influence the DNA repair capacity of lung cancer by regulating <em>PEX3</em> and <em>PHACTR2‑AS1</em> expression instead of <em>PHACTR2</em>". Oncology Reports 47.3 (2022): 59.
Chicago
Shi, Q., Shi, Q., Xu, J., Wang, H., Li, Y., Zhang, X., Fu, Y., Tian, R., Jiang, R., Liu, C., Sun, C."rs9390123 and rs9399451 influence the DNA repair capacity of lung cancer by regulating <em>PEX3</em> and <em>PHACTR2‑AS1</em> expression instead of <em>PHACTR2</em>". Oncology Reports 47, no. 3 (2022): 59. https://doi.org/10.3892/or.2022.8270